Počet záznamů: 1

The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase

  1. 1.
    0355844 - UEK-B 2011 RIV US eng J - Článek v odborném periodiku
    Kulik, Natallia - Weignerová, L. - Filipi, Tomáš - Pompach, Petr - Novák, Petr - Mrázek, H. - Slámová, Kristýna - Bezouška, K. - Křen, Vladimír - Ettrich, Rüdiger
    The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase.
    Glycobiology. Roč. 20, č. 11 (2010), s. 1410-1419 ISSN 0959-6658
    Grant CEP: GA MŠk(CZ) LC06010; GA ČR GAP207/10/1934; GA ČR GAP207/10/1040; GA ČR GA303/09/0477; GA ČR GAP207/10/0321
    Výzkumný záměr: CEZ:AV0Z60870520; CEZ:AV0Z50200510; CEZ:AV0Z10750506
    Klíčová slova: α-N-acetylgalactosaminidase * α-galactosidase * Aspergillus niger * substrate binding * molecular modeling
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 3.791, rok: 2010

    Two genes in the genome of Aspergillus niger, aglA and aglB, have been assigned to encode for α-d-galactosidases variant A and B. However, analyses of primary and 3D structures based on structural models of these two enzymes revealed significant differences in their active centers suggesting important differences in their specificity for the hydrolyzed carbohydrates. To test this unexpected finding, a large screening of libraries from 42 strains of filamentous fungi succeeded in identifying an enzyme from A. niger CCIM K2 that exhibited both α-galactosidase andα-N-acetylgalactosaminidase activities, with the latter activity predominating. The enzyme protein was sequenced, and its amino acid sequence could be unequivocally assigned to the enzyme encoded the aglA gene. Enzyme activity measurements and substrate docking clearly demonstrated the preference of the identified enzyme for α-N-acetyl-d-galactosaminide over α-d-galactoside.
    Trvalý link: http://hdl.handle.net/11104/0194522