Počet záznamů: 1

Laser Desorption-Ionization of Lipid Transfers: Tissue Mass Spectrometry Imaging without MALDI Matrix

  1. 1.
    0354297 - MBU-M 2011 RIV US eng J - Článek v odborném periodiku
    Vidová, V. - Novák, Petr - Strohalm, Martin - Pól, Jaroslav - Havlíček, Vladimír - Volný, Michael
    Laser Desorption-Ionization of Lipid Transfers: Tissue Mass Spectrometry Imaging without MALDI Matrix.
    Analytical Chemistry. Roč. 82, č. 12 (2010), s. 4994-4997 ISSN 0003-2700
    Grant CEP: GA MŠk LC545; GA MŠk LC07017; GA ČR GPP206/10/P018
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: ELECTROSPRAY-IONIZATION * METABOLITES * SECTIONS
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 5.874, rok: 2010

    Mass spectrometry imaging of tissue-lipid transfers without MALDI matrix is demonstrated. Commercially available nanostructured surfaces (nano-assisted laser desorption-ionization or NALDI) are used as substrates for imprinting of tissue sections. The lithographic transfers are then washed and the two-dimensional distribution of the lipids is imaged by laser desorption-ionization mass spectrometry. The NALDI imaging of lipid transfers is compared with standard MALDI imaging of matrix-coated tissue sections. The obtained images are of the same quality, and no spatial information is lost due to the imprinting process. NALDI imaging is faster due to the absence of the time-consuming matrix deposition step, and the NALDI mass spectra are less complex and easier to interpret than standard MALDI
    Trvalý link: http://hdl.handle.net/11104/0193334