Počet záznamů: 1

Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae

  1. 1.
    0354019 - FGU-C 2011 RIV US eng J - Článek v odborném periodiku
    Burdíková, Zuzana - Čapek, Martin - Ostašov, Pavel - Mitchell, E.A.D. - Machač, Jiří - Kubínová, Lucie
    Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae.
    Microscopy and Microanalysis. Roč. 16, Suppl.2 (2010), s. 1142-1143 ISSN 1431-9276.
    [Microscopy and Microanalysis 2010. Portland, 01.08.2010-05.08.2010]
    Grant CEP: GA MŠk(CZ) LC06063; GA ČR(CZ) GA102/08/0691; GA ČR(CZ) GA304/09/0733
    Výzkumný záměr: CEZ:AV0Z50110509; CEZ:AV0Z60050516
    Klíčová slova: testate amoebae * confocal microscopy * two-photon microscopy
    Kód oboru RIV: BH - Optika, masery a lasery
    Impakt faktor: 2.179, rok: 2010

    We applied CLSM and Two-Photon Excitation (TPE) microscopy techniques to visualize testate amoebae (TA), their test, morphology and physiology. TA are usually examined using SEM and E-SEM. It is not possible to acquire images of living TA and physiology of the amoeba inside the test by SEM or E-SEM. The goal of the study was to examine the potential of CLSM and TPE for imaging of TA. We tried 17 fluorescent dyes to label different structures of TA. CLSM enabled us to acquire images of TA from depths up to 40 μm, whereas TPE was able to penetrate to 60 μm. Stereological methods were employed to estimate the volume of the biomass of TA visualized by CLSM
    Trvalý link: http://hdl.handle.net/11104/0193112