Počet záznamů: 1

Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA

  1. 1.
    0353703 - UOCHB-X 2011 RIV US eng M - Část monografie knihy
    Macíčková-Cahová, Hana - Vrábel, Milan - Hocek, Michal
    Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA.
    Current Protocols in Chemical Biology. Vol. 2. New York: Wiley Interscience, 2010, s. 1-14. ISBN 9780470559277
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: nucleoside triphosphates * DNA polymerase * cross-coupling * DNA conjugates
    Kód oboru RIV: CC - Organická chemie

    A novel, efficient, two-step methodology is presented for construction of base-modified oligonucleotides or DNA involving aqueous cross-coupling reactions of halogenated nucleoside triphosphates (dNTPs) with terminal acetylenes or arylboronic acids, followed by polymerase incorporation of the modified dNTPs either using primer extension (PEX) or polymerase chain reaction (PCR).
    Trvalý link: http://hdl.handle.net/11104/0192869