Počet záznamů: 1

Analysis and three-dimensional visualization of collagen in artificial scaffolds using nonlinear microscopy techniques

  1. 1.
    0353572 - UEM-P 2011 RIV US eng J - Článek v odborném periodiku
    Filová, Eva - Burdíková, Zuzana - Rampichová, Michala - Bianchini, P. - Čapek, Martin - Košťáková, E. - Amler, Evžen - Kubínová, Lucie
    Analysis and three-dimensional visualization of collagen in artificial scaffolds using nonlinear microscopy techniques.
    Journal of Biomedical Optics. Roč. 15, č. 6 (2010), 066011-1-066011-7 ISSN 1083-3668
    Grant CEP: GA MŠk(CZ) LC06063; GA ČR GAP304/10/1307; GA AV ČR IAA500390702
    Grant ostatní: GA MŠk(CZ) MSM6840770022; GA MŠk(CZ) 2B06130; GA ČR(CZ) GA102/08/0691; GA MŠk(CZ) 1M0510; GA ČR(CZ) GA202/09/1151; EU(XE) BIOSCENT ID 214539
    Výzkumný záměr: CEZ:AV0Z50390512; CEZ:AV0Z50390703; CEZ:AV0Z50110509
    Klíčová slova: biomedical materials * biomedical optical imaging * cellular biophysics
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 3.188, rok: 2010

    Extracellularly distributed collagen and chondrocytes seeded in gelatine and poly-ɛ-caprolactone scaffolds are visualized by two-photon excitation microscopy and second-harmonic generation . Joint application of TPEM and SHG imaging in combination with stereological measurements of collagen enables us not only to take high-resolution 3-D images, but also to quantitatively analyze the collagen volume and a spatial arrangement of cell-collagen-scaffold systems, which was previously impossible. This novel approach represents a powerful tool for the analysis of collagen-containing scaffolds with applications in cartilage tissue engineering.
    Trvalý link: http://hdl.handle.net/11104/0192796