Počet záznamů: 1

The transmembrane adaptor protein NTAL signals to mast cell cytoskeleton via the small GTPase Rho

  1. 1.
    0353421 - UMG-J 2011 RIV DE eng J - Článek v odborném periodiku
    Tůmová, Magda - Koffer, Anna - Šimíček, Michal - Dráberová, Lubica - Dráber, Petr
    The transmembrane adaptor protein NTAL signals to mast cell cytoskeleton via the small GTPase Rho.
    European Journal of Immunology. Roč. 40, č. 11 (2010), s. 3235-3245 ISSN 0014-2980
    Grant CEP: GA MŠk 1M0506; GA MŠk LC545; GA ČR(CZ) GD204/05/H023; GA ČR GA301/09/1826; GA ČR GAP302/10/1759; GA AV ČR KAN200520701
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: cell activation * cytoskeleton * mast cells
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.942, rok: 2010

    The transmembrane adaptor protein NTAL participates in signalosome assembly in hematopoietic cells, but its exact role in cell physiology remains enigmatic. This study shows that mast cells from NTAL-deficient mice, responding to Ag alone or in combination with SCF, exhibit reduced spreading on fibronectin, enhanced filamentous actin depolymerization and enhanced migration towards Ag relative to WT cells. No such differences between WT and NTAL(-/-) cells were observed when SCF alone was used as activator. Next the activities of two small GTPases, Rac and Rho, which are important regulators of actin polymerization were examined. Stimulation with Ag and/or SCF enhanced activity of Rac (1,2,3) in both NTAL(-/-) and WT cells. In contrast, RhoA activity decreased and this trend was much faster and more extensive in NTAL(-/-) cells, indicating a positive regulatory role of NTAL in the recovery of RhoA activity.
    Trvalý link: http://hdl.handle.net/11104/0192674
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