Počet záznamů: 1

Structure analysis of group I plant nucleases

  1. 1.
    0353312 - UMCH-V 2011 RIV DK eng J - Článek v odborném periodiku
    Dohnálek, Jan - Kovaľ, Tomáš - Lipovová, P. - Podzimek, Tomáš - Matoušek, Jaroslav
    Structure analysis of group I plant nucleases.
    Journal of Synchrotron Radiation. Roč. 18, Part 1 (2011), s. 29-30 ISSN 0909-0495.
    [International Symposium on Diffraction Structural Biology /3./. Paris - Orsay, 25.05.2010-28.05.2010]
    Grant CEP: GA ČR GA310/09/1407; GA ČR GA521/09/1214
    Výzkumný záměr: CEZ:AV0Z40500505; CEZ:AV0Z10100521; CEZ:AV0Z50510513
    Klíčová slova: nuclease * protein structure * crystal structure
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 2.726, rok: 2011

    Anticancer drugs attacking nucleic acids of the target cells have so far been based on animal or fungal ribonucleases. Plant nucleases have been proved to exhibit decreased cytotoxic side effects. Tomato bifunctional nuclease 1 with activity against both single-stranded and double-stranded RNA and DNA was produced in tobacco leaves as recombinant protein. The enzyme crystallizes under several different crystallization conditions. The presence of Zn2+ ions was confirmed by X-ray fluorescence. First crystallographic data were obtained.
    Trvalý link: http://hdl.handle.net/11104/0192591