Počet záznamů: 1

Structure of laccase from Streptomyces coelicolor after soaking with potassium hexacyanoferrate and at an improved resolution of 2.3 Å

  1. 1.
    0353303 - UMCH-V 2011 RIV GB eng J - Článek v odborném periodiku
    Skálová, Tereza - Dušková, Jarmila - Hašek, Jindřich - Štěpánková, Andrea - Kovaľ, Tomáš - Ostergaard, L. H. - Dohnálek, Jan
    Structure of laccase from Streptomyces coelicolor after soaking with potassium hexacyanoferrate and at an improved resolution of 2.3 Å.
    Acta Crystallographica Section F. Roč. 67, č. 1 (2011), s. 27-32 ISSN 1744-3091
    Grant CEP: GA ČR GA305/07/1073; GA AV ČR IAA500500701
    Výzkumný záměr: CEZ:AV0Z40500505; CEZ:AV0Z10100521
    Klíčová slova: laccase * potassium hexacyanoferrate * X-ray diffraction
    Kód oboru RIV: CD - Makromolekulární chemie
    Impakt faktor: 0.506, rok: 2011

    The paper reports the structure of the small laccase from Streptomyces coelicolor determined from a crystal soaked with potassium hexacyanoferrate [K4Fe(CN)6]. The decolorization of the natively blue crystal observed upon soaking indicates the reduction of the enzyme in the crystal. The ligand binds between laccase molecules and stabilizes the crystal. The increased diffraction limit of the diffraction data collected from this crystal enabled the refinement of the small laccase structure at 2.3 Å resolution, which is the highest resolution obtained to date.
    Trvalý link: http://hdl.handle.net/11104/0192586