Počet záznamů: 1

Bordetella Adenylate Cyclase Toxin Mobilizes Its beta(2) Integrin Receptor into Lipid Rafts to Accomplish Translocation across Target Cell Membrane in Two Steps

  1. 1.
    0352882 - MBU-M 2011 RIV US eng J - Článek v odborném periodiku
    Bumba, Ladislav - Mašín, Jiří - Fišer, R. - Šebo, Peter
    Bordetella Adenylate Cyclase Toxin Mobilizes Its beta(2) Integrin Receptor into Lipid Rafts to Accomplish Translocation across Target Cell Membrane in Two Steps.
    P L o S Pathogens. Roč. 6, č. 5 (2010), s. 1-15 ISSN 1553-7366
    Grant CEP: GA ČR GP310/07/P115; GA MŠk 1M0506; GA AV ČR IAA500200914; GA MŠk 2B06161; GA ČR GA310/08/0447
    Výzkumný záměr: CEZ:AV0Z50200510; CEZ:AV0Z50520701
    Klíčová slova: SHEEP ERYTHROCYTES * BACILLUS-ANTHRACIS * ESCHERICHIA-COLI
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 9.079, rok: 2010

    The adenylate cyclase toxin (CyaA) of pathogenic Bordetellae eliminates the first line of host innate immune defense. It penetrates myeloid phagocytes, such as neutrophils, macrophage or dendritic cells, and subverts their signaling by catalyzing an extremely rapid conversion of intracellular ATP to the key signaling molecule cAMP. This efficiently inhibits the oxidative burst and complement- mediated opsonophagocytic killing of bacteria, thus enabling the pathogen to colonize host airways. We show that translocation of CyaA into phagocyte cytosol occurs in two steps. The toxin first binds the integrin CD11b/CD18 and inserts into phagocyte membrane to mediate influx of calcium ions into cells. This promotes relocation of the toxin-receptor complex into specific lipid microdomains within cell membrane called rafts
    Trvalý link: http://hdl.handle.net/11104/0192276