Počet záznamů: 1

Biotransformations with nitrilases

  1. 1.
    0352304 - MBU-M 2011 RIV GB eng J - Článek v odborném periodiku
    Martínková, Ludmila - Křen, Vladimír
    Biotransformations with nitrilases.
    Current Opinion in Chemical Biology. Roč. 14, č. 2 (2010), s. 130-137 ISSN 1367-5931
    Grant CEP: GA AV ČR IAA500200708; GA MŠk(CZ) LC06010; GA MŠk OC09046; GA MPO FT-TA5/043
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: ENZYME-PROMOTED DESYMMETRISATION * PSEUDOMONAS-FLUORESCENS EBC191 * SUBSTRATE-SPECIFICITY
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 9.312, rok: 2010

    The range of possible nitrilase applications has been recently broadened but in most cases the parameters of the reactions need to be improved to establish viable industrial processes. To achieve this goal, several methods have been used, primarily in screening for enzymes from new sources, enzyme improvement, substrate structure modification, medium engineering, and variation in process parameters. New nitrilases have been obtained by for example genome mining and the metagenomic approach. Protein engineering revealed targets for altering substrate specificity, activity or enantioselectivity, and for changing the acid:amide ratio in the nitrilase product
    Trvalý link: http://hdl.handle.net/11104/0191839