Počet záznamů: 1

Conserved mechanism of Wnt signaling function in the specification of vulval precursor fates in C. elegans and C. briggsae

  1. 1.
    0352047 - BC-A 2011 RIV US eng J - Článek v odborném periodiku
    Seetharaman, A. - Cumbo, P. - Bojanala, Nagagireesh - Gupta, B. P.
    Conserved mechanism of Wnt signaling function in the specification of vulval precursor fates in C. elegans and C. briggsae.
    Developmental Biology. Roč. 346, č. 1 (2010), s. 128-139 ISSN 0012-1606
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: Nematode * C. elegans * C. briggsae * Vulval development * Signal transduction * Wnt signaling
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.094, rok: 2010

    To understand the function and evolution of Wnt signaling in Caenorhabditis nematodes we focused on C. briggsae, a species that is substantially divergent from C. elegans in terms of the evolutionary time scale yet shares almost identical morphology. We isolated mutants in C briggsae that display multiple pseudo-vulvae resulting from ectopic VPC induction. We cloned one of these loci and found that it encodes an Axin homolog, Cbr-PRY-1. Our genetic studies revealed that Cbr-pry-1 functions upstream of the canonical Wnt pathway components Cbr-bar-1 (beta-catenin) and Cbr-pop-1(tcf/lef) as well as the Hox target Cbr-lin-39 (Dfd/Scr). We further characterized the pry-1 vulval phenotype in C briggsae and C elegans using 8 cell fate markers, cell ablation, and genetic interaction approaches. Our results show that ectopically induced VPCs in pry-1 mutants adopt 2 degrees fates independently of the gonad-derived inductive and LIN-12/Notch-mediated lateral signaling pathways.
    Trvalý link: http://hdl.handle.net/11104/0191649