Počet záznamů: 1

Access to enantiopure aromatic epoxides and diols using epoxide hzdrolases derived from total biofilter DNA

  1. 1.
    0351499 - MBU-M 2011 RIV NL eng J - Článek v odborném periodiku
    Kotík, Michael - Štěpánek, Václav - Grulich, Michal - Kyslík, Pavel - Archelas, A.
    Access to enantiopure aromatic epoxides and diols using epoxide hzdrolases derived from total biofilter DNA.
    Journal of Molecular Catalysis B-Enzymatic. Roč. 65, 1-4 (2010), s. 41-48 ISSN 1381-1177
    Grant CEP: GA ČR GA204/06/0458
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: Chiral resolution * Enantioconvergent reaction * Enantioselectivity
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 2.330, rok: 2010

    Two genes encoding functional epoxide hydrolases were retrieved from total DNA isolated from biofilter-derived biomass, using PCR with epoxide hydrolase-specific degenerate primers followed by genome-walking PCR. The resulting recombinant epoxide hydrolases were expressed in Escherichia coli and characterised
    Trvalý link: http://hdl.handle.net/11104/0191238