Počet záznamů: 1

High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula

  1. 1.
    0351441 - MBU-M 2011 RIV NL eng J - Článek v odborném periodiku
    Stodůlková, Eva - Man, Petr - Kolařík, Miroslav - Flieger, Miroslav
    High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula.
    Journal of Chromatography A. Roč. 1217, č. 40 (2010), s. 6296-6302 ISSN 0021-9673
    Grant CEP: GA MŠk 2B08064; GA AV ČR KAN200200651
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: High-performance liquid chromatography * Mass spectrometry * Anthraquinones
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 4.194, rok: 2010

    Lilac coloured species of Geosmithia lavendula produce a mixture of polyhydroxylated anthraquinones under condition of submerged fermentation. Three pigments had been isolated and identified earlier as a 1,3,6,8-tetrahydroxyanthraquinone (compound 7), rhodolamprometrin (1-acetyl 2,4,5,7-tetrahydroxyanthraquinone; compound 5), and 1-acetyl 2,4,5,7,8-penthahydroxyanthraquinone (compound 4). A new HPLC method was developed for the separation of three known and ten new anthraquinone pigments. In addition, five new pigments were determined by FTMS as coeluting impurities. The analyses were performed on a reversed phase column using gradient elution with a mobile phase system consisting of phosphate buffer (50mM, pH 2.0) and acetonitrile. The structure evaluation was based namely on FTMS and UV–VIS spectrometry. The developed procedure was used for the determination of individual anthraquinones in fermentation broth of G. lavendula after 14 days of cultivation
    Trvalý link: http://hdl.handle.net/11104/0191196