Počet záznamů: 1

Novel antimicrobial peptides from the venom of the eusocial bee Halictus sexcinctus (Hymenoptera: Halictidae) and their analogs

  1. 1.
    0351367 - UOCHB-X 2011 RIV AT eng J - Článek v odborném periodiku
    Monincová, Lenka - Buděšínský, Miloš - Slaninová, Jiřina - Hovorka, Oldřich - Cvačka, Josef - Voburka, Zdeněk - Fučík, Vladimír - Borovičková, Lenka - Bednárová, Lucie - Straka, J. - Čeřovský, Václav
    Novel antimicrobial peptides from the venom of the eusocial bee Halictus sexcinctus (Hymenoptera: Halictidae) and their analogs.
    Amino Acids. Roč. 39, č. 3 (2010), s. 763-775 ISSN 0939-4451
    Grant CEP: GA ČR GA203/08/0536
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: antimicrobial peptides * Wild-bee venom * hemolytic activity * NMR spectroscopy * CD spectroscopy
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 4.106, rok: 2010

    Two novel antimicrobial peptides Gly-Met-Trp-Ser-Lys-Ile-Leu-Gly-His-Leu-Ile-Arg-NH2 (HAL-1) and Gly-Lys-Trp-Met-Ser-Leu-Leu-Lys-His-Ile-Leu-Lys-NH2 (HAL-2) designated halictines were identified in the venom of the wild bee Halictus sexcinctus. They possess high antimicrobial activity against both Gram-positive and -negative bacteria and noticeable hemolytic activity. As indicated by CD and NMR spectroscopy, halictines can adopt an amphipathic alpha-helical secondary structure in an anisotropic environment such as the bacterial cell membrane. Altogether we prepared 51 of HAL-1 and HAL-2 analogs to study the effect of such structural parameters as cationicity, hydrophobicity, alpha-helicity, amphipathicity, and truncation on antimicrobial and hemolytic activities.
    Trvalý link: http://hdl.handle.net/11104/0191135