Počet záznamů: 1

Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus

  1. 1.
    0351320 - UMG-J 2011 RIV DE eng J - Článek v odborném periodiku
    Philimonenko, Vlada - Janáček, Jiří - Harata, M. - Hozák, Pavel
    Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus.
    Histochemistry and Cell Biology. Roč. 134, č. 3 (2010), s. 243-249 ISSN 0948-6143
    Grant CEP: GA ČR(CZ) GA204/07/1592; GA MŠk 2B06063; GA MŠk LC545; GA MŠk ME09101
    Výzkumný záměr: CEZ:AV0Z50520514; CEZ:AV0Z50110509
    Klíčová slova: RNA-polymerase-I * ultrastructural analysis * UBF
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.727, rok: 2010

    Nuclear actin and nuclear myosin I (NMI) are important players in transcription of ribosomal genes. Transcription of rDNA takes place in highly organized intranuclear compartment, the nucleolus. In this study, we characterized the localization of these two proteins within the nucleolus of HeLa cells with high structural resolution by means of electron microscopy and gold-immunolabeling. We demonstrate that both actin and NMI are localized in specific compartments within the nucleolus, and the distribution of NMI is transcription-dependent. Moreover, a pool of NMI is present in the foci containing nascent rRNA transcripts. Actin, in turn, is present both in transcriptionally active and inactive regions of the nucleolus and colocalizes with RNA polymerase I and UBF. Our data support the involvement of actin and NMI in rDNA transcription and point out to other functions of these proteins in the nucleolus, such as rRNA maturation and maintenance of nucleolar architecture.
    Trvalý link: http://hdl.handle.net/11104/0191099
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