Počet záznamů: 1

Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance biosensor study

  1. 1.
    0350303 - URE-Y 2011 RIV GB eng J - Článek v odborném periodiku
    Špringer, Tomáš - Šípová, Hana - Vaisocherová, Hana - Štepánek, J. - Homola, Jiří
    Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance biosensor study.
    Nucleic Acids Research. Roč. 38, č. 20 (2010), s. 7343-7351 ISSN 0305-1048
    Grant CEP: GA AV ČR KAN200670701
    Grant ostatní: Univerzita Karlova(CZ) SVV-2010-261 304
    Výzkumný záměr: CEZ:AV0Z20670512
    Klíčová slova: oligonucleotide * monovalent and divalent cations * surface plasmon resonance
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 7.836, rok: 2010

    We investigated the simultaneous effect of monovalent and divalent cations on hybridization of DNA targets to DNA probes immobilized on the surface of a surface plasmon resonance sensor. Our results demonstrated that divalent magnesium is found to be much more efficient in duplex stabilization than monovalent sodium. This trend is opposite to that established for oligonucleotides in a solution. Moreover, the selected saline composition of buffer improved the discrimination of complementary and point mismatched DNA targets.
    Trvalý link: http://hdl.handle.net/11104/0190338
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