Počet záznamů: 1
Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement
0349037 - UFCH-W 2011 RIV GB eng J - Článek v odborném periodiku
Humpolíčková, Jana - Benda, Aleš - Macháň, Radek - Enderlein, J. - Hof, Martin
Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement.
Physical Chemistry Chemical Physics. Roč. 12, č. 39 (2010), s. 12457-12465 ISSN 1463-9076
Grant CEP: GA MŠk(CZ) LC06063; GA AV ČR KJB400400904; GA AV ČR GEMEM/09/E006
Výzkumný záměr: CEZ:AV0Z40400503
Klíčová slova: fluorescence microscopy * dunamic saturation optical microscopy * fluorescence
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 3.454, rok: 2010
Fluorescence microscopy has become one of the most rapidly developing observation techniques in the field of molecular biology, since its high sensitivity, contrast and labeling specificity together with being non-invasive fulfill the most important requirements of live cell imaging. The biggest limitation of the technique seems to be the spatial resolution which is, based on Abbe's diffraction law, restricted to some hundreds of nanometres. Recently, various approaches have been developed that overcome the limit imposed by the diffraction barrier and these methods currently lead the development in the field of fluorescence microscopy. In this contribution, we present dynamic saturation optical microscopy (DSOM) a new technique that monitors the temporal decay of the excited singlet state due to a dark state formation. By mapping the intensity dependent decay kinetics, enhanced resolution images can be obtained.
Trvalý link: http://hdl.handle.net/11104/0189381