Počet záznamů: 1

Autocrine effects of visfatin on hepatocyte sensitivity to insulin action

  1. 1.
    0347884 - FGU-C 2011 RIV CZ eng J - Článek v odborném periodiku
    Škop, V. - Kontrová, K. - Zídek, Václav - Pravenec, Michal - Kazdová, L. - Mikulík, Karel - Sajdok, J. - Zídková, J.
    Autocrine effects of visfatin on hepatocyte sensitivity to insulin action.
    Physiological Research. Roč. 59, č. 4 (2010), s. 615-618 ISSN 0862-8408
    Grant CEP: GA AV ČR(CZ) IAA500110805; GA MŠk(CZ) ME08006; GA MZd(CZ) NR9387; GA MZd(CZ) NR9359
    Výzkumný záměr: CEZ:AV0Z50110509; CEZ:AV0Z50200510
    Klíčová slova: Visfatin * RNA interference * insulin mimetic effects
    Kód oboru RIV: FB - Endokrinologie, diabetologie, metabolizmus, výživa
    Impakt faktor: 1.646, rok: 2010

    Visfatin was originally described as an adipokine with insulin mimetic effects. Since liver plays a major role in glucose metabolism, we studied visfatin effects on insulin-stimulated cellular glucose uptake in Fao rat hepatocytes using RNA interference (RNAi). RNAi-mediated downregulation of visfatin expression in Fao cells was associated with significantly reduced NAD biosynthesis (0.3+/-0.01 vs. 0.5+/-0.01 mmol/h/g, P<0.05) and with significantly decreased incremental glucose uptake after stimulation with insulin when compared to controls with normal expression of visfatin (0.6+/-0.2 vs. 2.2+/-0.5 nnmol/g/2 h, P=0.02). These results provide evidence that visfatin exhibits important autocrine effects on sensitivity of liver cells to insulin action possibly through its effects on NAD biosynthesis
    Trvalý link: http://hdl.handle.net/11104/0188557