Počet záznamů: 1

Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point

  1. 1.
    0347130 - BU-J 2011 RIV NL eng J - Článek v odborném periodiku
    Bukovská, Petra - Jelínková, Markéta - Hršelová, Hana - Sýkorová, Zuzana - Gryndler, Milan
    Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point.
    Journal of Microbiological Methods. Roč. 82, č. 3 (2010), s. 223-228 ISSN 0167-7012
    Grant CEP: GA MŠk 1M0571
    Výzkumný záměr: CEZ:AV0Z60050516; CEZ:AV0Z50200510
    Klíčová slova: T-RFLP * drift * DNA fragment
    Kód oboru RIV: EF - Botanika
    Impakt faktor: 2.018, rok: 2010

    Several methods of molecular analysis of microbial diversity, including terminal restriction fragment length polymorphism (T-RFLP) analysis are based on measurement of the DNA fragment length. Significant variation between sequence-determined and measured length of restriction fragments (drift) has been observed, which can affect the efficiency of the identification of microorganisms in the analyzed communities. In this study, principal component analysis and multiple regression analysis revealed that secondary structure melting point and G+C nucleotide content, besides the fragment length, contribute to the variation observed, whereas the contribution of purine
    Trvalý link: http://hdl.handle.net/11104/0187975