Počet záznamů: 1
Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point
- 1.0347130 - BÚ 2011 RIV NL eng J - Článek v odborném periodiku
Bukovská, Petra - Jelínková, Markéta - Hršelová, Hana - Sýkorová, Zuzana - Gryndler, Milan
Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point.
Journal of Microbiological Methods. Roč. 82, č. 3 (2010), s. 223-228. ISSN 0167-7012. E-ISSN 1872-8359
Grant CEP: GA MŠMT 1M0571
Výzkumný záměr: CEZ:AV0Z60050516; CEZ:AV0Z50200510
Klíčová slova: T-RFLP * drift * DNA fragment
Kód oboru RIV: EF - Botanika
Impakt faktor: 2.018, rok: 2010
Several methods of molecular analysis of microbial diversity, including terminal restriction fragment length polymorphism (T-RFLP) analysis are based on measurement of the DNA fragment length. Significant variation between sequence-determined and measured length of restriction fragments (drift) has been observed, which can affect the efficiency of the identification of microorganisms in the analyzed communities. In this study, principal component analysis and multiple regression analysis revealed that secondary structure melting point and G+C nucleotide content, besides the fragment length, contribute to the variation observed, whereas the contribution of purine
Trvalý link: http://hdl.handle.net/11104/0187975
Počet záznamů: 1