Počet záznamů: 1

Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices

  1. 1.
    0346926 - BTO-N 2011 RIV US eng J - Článek v odborném periodiku
    Coll, A. - Nadal, A. - Collado, R. - Capellades, G. - Kubista, Mikael - Messeguer, J. - Pla, M.
    Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices.
    Plant Molecular Biology. Roč. 73, č. 3 (2010), s. 349-362 ISSN 0167-4412
    Výzkumný záměr: CEZ:AV0Z50520701
    Klíčová slova: GMO (Genetically Modified Organism) * MON810 * Maize * Transcriptome
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.149, rok: 2010

    Over the last few years, targeted approaches have been complemented by profiling methods to assess possible unintended effects of transformation of GMO. Here we used Affymetrix microarray platform to evaluate transcriptional differences between commercial MON810 GM maize and non-transgenic crops. We took two common MON810/non-GM variety pairs and two farming practices. MON810 and comparable non-GM varieties have very low numbers of sequences with differential expression. We show, the differences between a given MON810 variety and the non-GM do not depend on the assayed cultural conditions. Natural variation explained most of the variability in gene expression. Up to 37 % was dependent upon the variety and 32 % a result of the fertilization. The MON810 had minor effect (9.7%) on gene expression. This indicates that transcriptional differences of conventionally-bred varieties and different environmental conditions should be taken into account in safety assessment studies of GM plants.
    Trvalý link: http://hdl.handle.net/11104/0187826