Počet záznamů: 1

A lens-specific co-injection marker for medaka transgenesis

  1. 1.
    0346841 - UMG-J 2011 RIV US eng J - Článek v odborném periodiku
    Vopálenský, Pavel - Růžičková, Jana - Pavlů, Barbora - Kozmik, Zbyněk
    A lens-specific co-injection marker for medaka transgenesis.
    Biotechniques. Roč. 48, č. 3 (2010), s. 235-236 ISSN 0736-6205
    Grant CEP: GA AV ČR IAA500520908; GA MŠk 1M0520
    Grant ostatní:GA UK(CZ) 91808
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: lens * transgenic * crystallin
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 2.550, rok: 2010

    Recent advances in fish transgenesis allow generation of transgenic animals at high numbers and efficiency of germ-line transmission. To facilitate the procedure, a suitable co-injection marker may be very helpful for preselection of transgenic embryos. For this purpose, a lens-specific marker appears to be a suitable candidate since the lens is a well defined tissue positioned at the periphery and is easily accessible for examination of reporter gene expression. Here, we constructed reporter vectors based on mouse gamma-F crystallin (mF-Cry) promoter driving heterologous expression of the reporter gene at a high level and very specifically to the lens allowing an easy sorting of transgenics.
    Trvalý link: http://hdl.handle.net/11104/0187759