Počet záznamů: 1

Method for resolution and quantification of components of the non-photochemical quenching (qN)

  1. 1.
    0346771 - BC-A 2011 RIV NL eng J - Článek v odborném periodiku
    Roháček, Karel
    Method for resolution and quantification of components of the non-photochemical quenching (qN).
    Photosynthesis Research. Roč. 105, č. 2 (2010), s. 101-113 ISSN 0166-8595
    Grant CEP: GA AV ČR IAA600960716
    Výzkumný záměr: CEZ:AV0Z50510513
    Klíčová slova: Dark relaxation * Fluorescence quenching * Multi-exponential regression
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 2.410, rok: 2010

    A new method of the chlorophyll (Chl) fluorescence quenching analysis is described, which allows the calculation of values of (at least) three components of the non-photochemical quenching of the variable Chl a fluorescence (qN) using a non-linear regression of a multi-exponential function within experimental data. Formulae for coefficients of the “energy”-dependent quenching (qE), the state-transition quenching (qT) and the photo/inhibitory quenching (qI) of Chl a fluorescence were found on the basis of three assumptions: (i) the dark relaxation kinetics of qN, as well as of all its components, is of an exponential nature, (ii) the superposition principle is valid for individual Chl a fluorescence quenching processes, and (iii) the same reference fluorescence level (Fv) is used to define both qN and its components. All definitions as well as the algorithms for analytical recognition of the qN components are theoretically clarified and experimentally tested.
    Trvalý link: http://hdl.handle.net/11104/0187703