Počet záznamů: 1
Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L
0346619 - BTO-N 2011 RIV US eng J - Článek v odborném periodiku
Li, P. - Hulak, M. - Koubek, Pavek - Šulc, Miroslav - Dzyuba, B. - Boryshpolets, S. - Rodina, M. - Gela, D. - Maňásková-Postlerová, Pavla - Pěknicová, Jana - Linhart, O.
Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L.
Theriogenelogy. Roč. 74, č. 3 (2010), s. 413-423 ISSN 0093-691X
Výzkumný záměr: CEZ:AV0Z50520701; CEZ:AV0Z5020903
Klíčová slova: sperm quality * cryodamage * spermatozoa * seminal plasma * fish
Kód oboru RIV: EI - Biotechnologie a bionika
Impakt faktor: 2.045, rok: 2010
Damage to spermatozoa during cryopreservation is regarded as a major obstacle to the expansion of sperm storage technology. The authors used 2D PAA gel electrophoresis and MALDI-TOF to explore whether the protein profile of common carp (Cyprinus carpio) spermatozoa is affected by cryopreservation. 14 protein spots were significantly altered following cryopreservation. Eleven of these were identified: three as specific membrane proteins involved in membrane trafficking, organization, and cell movement; six as cytoplasmic enzymes involved in cell metabolism, oxidoreductase activity, and signal transduction; and transferrin. Based on these findings, the authors hypothesize that transferrin in cryopreserved sperm may protect spermatozoa against oxidative damage during the freeze-thaw process. Cryopreservation caused changes in spermatozoa protein profiles that may lead to decreased spermatozoa velocity, motility, fertilization success and to subsequent ova hatching rate.
Trvalý link: http://hdl.handle.net/11104/0187599