Počet záznamů: 1
Analysis of wax ester molecular species by high performance liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry
0345947 - UOCHB-X 2011 RIV NL eng J - Článek v odborném periodiku
Vrkoslav, Vladimír - Urbanová, Klára - Cvačka, Josef
Analysis of wax ester molecular species by high performance liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry.
Journal of Chromatography A. Roč. 1217, č. 25 (2010), s. 4184-4194 ISSN 0021-9673
Grant CEP: GA ČR GA203/09/0139
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: Beeswax * Branched esters * Human hair * Jojoba * Mass spectrometry
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 4.194, rok: 2010
High chromatographic resolution of wax esters (WEs) was achieved by non-aqueous reversed-phase liquid chromatography on a Nova-Pak C18 column by optimizing the acetonitrile/ethyl acetate mobile phase gradient. The retention behaviour of saturated, unsaturated and methyl-branched WEs was studied in this chromatographic system. The analytes were detected by atmospheric pressure chemical ionization mass spectrometry (APCI-MS). WEs provided simple full-scan spectra with abundant protonated molecules and low-intensity fragments. Collision-induced dissociation (CID) promoted identification of the WE molecular species. The responses of WEs were found to be dependent on the number of double bonds and on the alkyl-chain length. The HPLC/APCIMS was applied for the analysis of the WEs isolated from honeycomb beeswax, jojoba oil and human hair. Good agreement between reported results and the literature data was achieved, with several novel polyunsaturated WEs also being found.
Trvalý link: http://hdl.handle.net/11104/0187108