Počet záznamů: 1

Second harmonic imaging of chloroplasts using the two-photon laser scanning microscope

  1. 1.
    0343182 - UEK-B 2011 RIV GB eng J - Článek v odborném periodiku
    Reshak, Ali H - Sarafis, V. - Heintzmann, R.
    Second harmonic imaging of chloroplasts using the two-photon laser scanning microscope.
    Micron. Roč. 40, č. 3 (2009), s. 378-385 ISSN 0968-4328
    Výzkumný záměr: CEZ:AV0Z60870520
    Klíčová slova: 2PLSM * collagen * generatin * scattering * membrane * fibrils
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 1.626, rok: 2009

    Background: Second harmonic information reveals information about the structure of spatially oriented structures with an asymmetry. We study the second harmonic images of the grana and intergranal parts of chloroplasts in the leaves of the shade moss Plagiomnium affine. Results: A two-photon microscope with blocking filters to suppress fluorescence generated both forward and backward second harmonic illumination to be captured. In the starch free chloroplasts of the single cell layered laminae strong second harmonic generation (SHG) from the granal regions was emitted. Upon illumination the chloroplasts changed their orientation affecting the SHG signal. Chloroplast is reoriented upon illumination.
    Trvalý link: http://hdl.handle.net/11104/0185716