Počet záznamů: 1

Lipopolythiourea/DNA interaction: A biophysical study

  1. 1.
    0342982 - UFCH-W 2011 RIV NL eng J - Článek v odborném periodiku
    Kral, Teresa - Leblond, J. - Hof, Martin - Scherman, D. - Hersovici, J. - Mignet, N.
    Lipopolythiourea/DNA interaction: A biophysical study.
    Biophysical Chemistry. Roč. 148, 1-3 (2010), s. 68-73 ISSN 0301-4622
    Grant CEP: GA AV ČR IAA400400621; GA MŠk(CZ) LC06063
    Výzkumný záměr: CEZ:AV0Z40400503
    Klíčová slova: lipopolythiourea * FCS * plasmid DNA compaction * 31P NMR
    Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
    Impakt faktor: 2.108, rok: 2010

    Lipopolythioureas (LPT) are original non cationic systems representing an alternative to cationic lipids. Their high transfection efficiency prompted us to investigate further their biophysical properties, and in particular how thiourea lipids interact with DNA. The interaction of lipopolythiourea with DNA was investigated by fluorescence correlation microscopy (FCS). Influence of the lipid length and nature of the thiourea head on the thiourea/DNA interaction were studied. FCS revealed a strong interaction between lipopolythiourea and DNA, occurring at 1 equivalent of a thiourea lipid by a DNA phosphate group, and leading to a condensed plasmid state. From previous in vitro experiments, we could conclude that the lipid leading to the more condensed state of DNA was also the more efficient to transfect cells.
    Trvalý link: http://hdl.handle.net/11104/0185576