Počet záznamů: 1

Functions and cellular localization of cysteine desulfurase and selenocysteine lyase in Trypanosoma brucei

  1. 1.
    0342953 - BC-A 2011 RIV GB eng J - Článek v odborném periodiku
    Poliak, Pavel - Van Hoewyk, D. - Oborník, Miroslav - Zíková, Alena - Stuart, K. D. - Tachezy, J. - Pilon, M. - Lukeš, Julius
    Functions and cellular localization of cysteine desulfurase and selenocysteine lyase in Trypanosoma brucei.
    FEBS Journal. Roč. 277, č. 2 (2010), s. 383-393 ISSN 1742-464X
    Grant CEP: GA ČR GA204/09/1667
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: Fe–S cluster * mitochondrion * RNAi * selenoprotein * Trypanosoma
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 3.129, rok: 2010

    It was shown previously that the Nfs-like protein called Nfs from the parasitic protist Trypanosoma brucei is a genuine CysD. A second Nfs-like protein is encoded in the nuclear genome of T. brucei. We called this protein selenocysteine lyase (SCL) because phylogenetic analysis reveals that it is monophyletic with known eukaryotic selenocysteine lyases. The Nfs protein is located in the mitochondrion, whereas the SCL protein seems to be present in the nucleus and cytoplasm. Unexpectedly, downregulation of either Nfs or SCL protein leads to a dramatic decrease in both CysD and selenocysteine lyase activities concurrently in the mitochondrion and the cytosolic fractions. Because loss of Nfs causes a growth phenotype but loss of SCL does not, we propose that Nfs can fully complement SCL, whereas SCL can only partially replace Nfs under our growth conditions.
    Trvalý link: http://hdl.handle.net/11104/0185552