Počet záznamů: 1

Probing for primary functions of prohibitin in Trypanosoma brucei

  1. 1.
    0342888 - BC-A 2011 RIV AU eng J - Článek v odborném periodiku
    Týč, Jiří - Faktorová, Drahomíra - Kriegová, Eva - Jirků, Milan - Vávrová, Zuzana - Maslov, D. A. - Lukeš, Julius
    Probing for primary functions of prohibitin in Trypanosoma brucei.
    International Journal for Parasitology. Roč. 40, č. 1 (2010), s. 73-83 ISSN 0020-7519
    Grant CEP: GA ČR GA204/09/1667; GA AV ČR IAA500960705; GA ČR(CZ) GP204/06/P423
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: prohibitin * mitochondrion * morphology * mitochondrial translation
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 3.822, rok: 2010

    We exploit the tractable reverse genetics of Trypanosoma brucei, the causative agent of African sleeping sickness, in order to specifically analyse the function of PHB in this highly divergent eukaryote. Using inducible RNA interference (RNAi) we show that PHB1 is essential in T brucei, where it is confined to the cell's single mitochondrion forming a high molecular weight complex. PHB1 and PHB2 appear to be indispensible for mitochondrial translation. Their ablation leads to a decrease in mitochondrial membrane potential, however no effect on the level of reactive oxygen species was observed. Flagellates lacking either PHB1 or both PHB1 and PHB2 exhibit significant morphological changes of their organelle, most notably its inflation. Even long after the loss of the PHB proteins, mtDNA was unaltered and mitochondrial cristae remained present, albeit displaced to the periphery of the mitochondrion, which is in contrast to other eukaryotes.
    Trvalý link: http://hdl.handle.net/11104/0185497