Počet záznamů: 1

Intra-individual internal transcribed spacer 1 (ITS1) and ITS2 ribosomal sequence variation linked with multiple rDNA loci: A case of triploid Atractolytocestus huronensis, the monozoic cestode of common carp

  1. 1.
    0342847 - BC-A 2011 RIV AU eng J - Článek v odborném periodiku
    Kraľová-Hromadová, I. - Štefka, Jan - Špakulová, M. - Orosová, Martina - Bombarová, M. - Hanzelová, V. - Bazsalovicsová, E. - Scholz, Tomáš
    Intra-individual internal transcribed spacer 1 (ITS1) and ITS2 ribosomal sequence variation linked with multiple rDNA loci: A case of triploid Atractolytocestus huronensis, the monozoic cestode of common carp.
    International Journal for Parasitology. Roč. 40, č. 2 (2010), s. 175-181 ISSN 0020-7519
    Grant CEP: GA MŠk LC522; GA ČR GA524/08/0885
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: Fish parasites * Caryophyllidea * Ribosomal divergence * Karyotype * FISH
    Kód oboru RIV: GJ - Choroby a škůdci zvířat, veterinární medicína
    Impakt faktor: 3.822, rok: 2010

    Complete sequences of the ribosomal internal transcribed spacers (ITS1 and ITS2) and karyological characters of the monozoic (unsegmented) tapeworm Atractolytocestus huronensis Anthony, 1958 (Cestoda: Caryophyllidea) from Slovakia were analysed, revealing considerable intra-genomic variability and triploidy in all analysed specimens. In individual tapeworms, two to four ITS1 and three to four ITS2 sequence types were found. Divergent intra-genomic ITS copies were mostly induced by nucleotide substitutions and different numbers of short repetitive motifs within the sequence. In addition, triploidy was found to be a common feature of A. huronensis. The karyotype of Slovakian A. huronensis possesses three sets of chromosomes (3n = 24, n = 4m + 3st + 1 minute chromosome). Fluorescent in situ hybridisation (FISH) with a ssrDNA probe revealed two distinct rDNA clusters for each homologue of the triplet number 2.
    Trvalý link: http://hdl.handle.net/11104/0185467