Počet záznamů: 1

Functional analysis of the skin-swelling response to phytohaemagglutinin

  1. 1.
    0342466 - UBO-W 2011 RIV GB eng J - Článek v odborném periodiku
    Vinkler, Michal - Bainová, H. - Albrecht, Tomáš
    Functional analysis of the skin-swelling response to phytohaemagglutinin.
    Functional Ecology. Roč. 24, č. 5 (2010), s. 1081-1086 ISSN 0269-8463
    Grant CEP: GA ČR GA206/08/1281; GA ČR GA206/08/0640; GA MŠk LC06073
    Výzkumný záměr: CEZ:AV0Z60930519
    Klíčová slova: avian innate and adaptive immunity * ecoimmunology * ecological immunology * ecotoxicology * inflammatory response * PHA-induced hypersensitivity * phytohaemagglutinin * T-cell-mediated immunocompetence
    Kód oboru RIV: EG - Zoologie
    Impakt faktor: 4.645, rok: 2010

    The phytohaemagglutinin (PHA) skin-swelling test is widely used in immunoecology and ecotoxicology to estimate cell-mediated immunity presumed to reveal T-cell mediated immunocompetence. To investigate the mechanism triggering this response we have compared in zebra finch (Taeniopygia guttata) the immune responses to two PHA isolectins (PHA-L and PHA-E) differing in their biological properties and one control protein. This is the first study to show the importance of erythroagglutination in the development of an inflammatory response to PHA-P. Our results indicate that the skin-swelling test using PHA-P reliably mirrors the individual general proinflammatory potential. The immunological background of the test is highly complex and the test results cannot be interpreted as measurements of the adaptive immunity or T-cell activity. This interpretational change importantly alters our view on the test results regarding the costs of the response or the evolutionary immunological adaptations.
    Trvalý link: http://hdl.handle.net/11104/0185197