Počet záznamů: 1

Direct polymerase synthesis of reactive aldehyde-functionalized DNA and its conjugation and staining with hydrazines

  1. 1.
    0342374 - UOCHB-X 2011 RIV DE eng J - Článek v odborném periodiku
    Raindlová, Veronika - Pohl, Radek - Šanda, Miloslav - Hocek, Michal
    Direct polymerase synthesis of reactive aldehyde-functionalized DNA and its conjugation and staining with hydrazines.
    Angewandte Chemie - International Edition. Roč. 49, č. 6 (2010), s. 1064-1066 ISSN 1433-7851
    Grant CEP: GA MŠk LC512; GA ČR GA203/09/0317
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: aldehydes * nucleotides * nucleoside triphosphates * DNA polymerase * DNA conjugates
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 12.730, rok: 2010

    Reactive aldehyde-modified DNA was prapered in two steps by cross-coupling reactions of halogenated dNTPs with 4-formylthiophene-2-boronic acid followed by polymerase (PEX or PCR) incorporations of the modified nucleotides to DNA. Aqueous hydrazone-formation was used in conjugation with aryl hydrazines for DNA staining.
    Trvalý link: http://hdl.handle.net/11104/0185124