Počet záznamů: 1

Asparagus officinalis: A New Host of 'Candidatus Phytoplasma asteris'

  1. 1.
    0342115 - BC-A 2011 RIV DE eng J - Článek v odborném periodiku
    Fránová, Jana - Petrzik, Karel
    Asparagus officinalis: A New Host of 'Candidatus Phytoplasma asteris'.
    Journal of Phytopathology - Phytopathologische Zeitschrift. Roč. 158, č. 4 (2010), s. 317-320 ISSN 0931-1785
    Grant CEP: GA AV ČR(CZ) 1QS500510558
    Výzkumný záměr: CEZ:AV0Z50510513
    Klíčová slova: 16S Ribosomal-RNA * mycoplasmalike organisms * gene-sequences
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 0.937, rok: 2010

    Asparagus officinalis plants with severe fasciation of some spears were observed in southern Bohemia between 1998 and 2007. Nucleic acids extracted from these and asymptomatic plants were assayed with nested polymerase chain reaction (PCR) using the phytoplasma-specific universal ribosomal primers P1/P7 and R16F2n/R2. The restriction profiles obtained from digestion of the PCR products with five endonucleases (AluI, HhaI, KpnI, MseI and RsaI) were identical in all phytoplasmas infecting asparagus in the Czech Republic and indistinguishable from those of phytoplasmas in the aster yellows group (subgroup 16SrI-B). Sequence analysis of 1754 bp of the ribosomal operon indicated that the closest related phytoplasmas were those associated with epilobium phyllody and onion yellows. This is the first report of the natural occurrence of 'Candidatus Phytoplasma asteris' in A. officinalis.
    Trvalý link: http://hdl.handle.net/11104/0184936