Počet záznamů: 1

The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod

  1. 1.
    0341849 - FGU-C 2011 RIV US eng J - Článek v odborném periodiku
    Čebašek, V. - Eržen, I. - Vyhnal, Aleš - Janáček, Jiří - Ribarič, S. - Kubínová, Lucie
    The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod.
    Microvascular Research. Roč. 79, č. 1 (2010), s. 40-46 ISSN 0026-2862
    Grant CEP: GA MŠk(CZ) MEB090910; GA MŠk(CZ) LC06063; GA ČR(CZ) GD204/09/H084
    Výzkumný záměr: CEZ:AV0Z50110509
    Klíčová slova: confocal microscopy * capillaries * length measurement
    Kód oboru RIV: EA - Morfologické obory a cytologie
    Impakt faktor: 2.390, rok: 2010

    We compared two methods of evaluation of capillary supply of individual skeletal muscle fibers, the traditional one using 2D images of thin transverse sections by the number of capillary profiles around a fiber (CAF) and the 3D approach where the mean length of capillaries around individual muscle fibers per fiber length (Lcap/Lfib) is measured from 3D images acquired by confocal microscopy. In models we showed that Lcap/Lfib was sensitive to different arrangements of capillaries, while CAF underestimated capillarization since it could not detect the increased length of capillary bed. In true muscle samples, we detected statistically significant differences in the capillary supply of control and denervated rat soleus muscles by both 2D and 3D methods. Lcap/Lfib was larger than CAF in control muscles reflecting their more complicated capillary bed
    Trvalý link: http://hdl.handle.net/11104/0184710