Počet záznamů: 1

Proteome Mining of Human Follicular Fluid Reveals a Crucial Role of Complement Cascade and Key Biological Pathways in Women Undergoing in Vitro Fertilization

  1. 1.
    0341837 - UZFG-Y 2011 RIV US eng J - Článek v odborném periodiku
    Jarkovská, Karla - Martinková, Jiřina - Lišková, Lucie - Halada, Petr - Moos, J. - Rezábek, K. - Gadher, S. J. - Kovářová, Hana
    Proteome Mining of Human Follicular Fluid Reveals a Crucial Role of Complement Cascade and Key Biological Pathways in Women Undergoing in Vitro Fertilization.
    Journal of Proteome Research. Roč. 9, č. 3 (2010), s. 1289-1301 ISSN 1535-3893
    Grant CEP: GA MZd(CZ) NS9781; GA AV ČR 1QS500450568
    Výzkumný záměr: CEZ:AV0Z50450515; CEZ:AV0Z50200510
    Klíčová slova: Human follicular fluid * plasma * assisted reproduction
    Kód oboru RIV: FK - Gynekologie a porodnictví
    Impakt faktor: 5.460, rok: 2010

    The primary aim of this study was to search for specific differences in protein composition of human follicular fluid (HFF) and plasma in order to identify proteins that accumulate or are absent in HFF. Depletion of abundant proteins combined with multidimensional protein fractionation allowed the study of middle- and lower-abundance proteins. Paired comparison study examining HFF with plasma/serum from women undergoing successful IVF revealed important differences in the protein composition which may improve our knowledge of the follicular microenvironment and its biological role. This study showed involvement of innate immune function of complement cascade in HFF Complement inhibition and the presence of C-terminal fragment of perlecan suggested possible links to angiogenesis which is a vital process in folliculogenesis and placental development. Differences in proteins associated with blood coagulation were also found in the follicular milieu.
    Trvalý link: http://hdl.handle.net/11104/0184701