Počet záznamů: 1

Preparative divergent flow IEF without carrier ampholytes for separation of complex biological samples

  1. 1.
    0338379 - UIACH-O 2010 RIV DE eng J - Článek v odborném periodiku
    Šťastná, Miroslava - Šlais, Karel
    Preparative divergent flow IEF without carrier ampholytes for separation of complex biological samples.
    Electrophoresis. Roč. 31, č. 3 (2010), s. 433-439 ISSN 0173-0835
    Grant CEP: GA AV ČR IAAX00310701
    Výzkumný záměr: CEZ:AV0Z40310501
    Klíčová slova: carrier-free divergent flow IEF * proteins * yeast lysate
    Kód oboru RIV: CB - Analytická chemie, separace
    Impakt faktor: 3.569, rok: 2010

    Based on the principles of recently newly established electrophoretic method called divergent flow IEF (DF IEF), we have tested the DF IEF instrument operating without the use of background carrier ampholytes. In DF IEF, the proteins are separated, desalted and concentrated in one step. We have shown that during separation and focusing of sample consisting of high numbers of proteins (yeast lysate and wheat flour extract), the pH gradient of preparative divergent flow IEF (DF IEF) can be created by autofocusing of the sample components themselves.
    Trvalý link: http://hdl.handle.net/11104/0182167