Počet záznamů: 1

Testing of the influenza virus purification by CIEF

  1. 1.
    0337690 - UIACH-O 2010 RIV DE eng J - Článek v odborném periodiku
    Horká, Marie - Kubíček, O. - Kubesová, Anna - Kubíčková, Z. - Rosenbergová, K. - Šlais, Karel
    Testing of the influenza virus purification by CIEF.
    Electrophoresis. Roč. 31, č. 2 (2010), s. 331-338 ISSN 0173-0835
    Grant CEP: GA AV ČR IAAX00310701
    Výzkumný záměr: CEZ:AV0Z40310501
    Klíčová slova: capillary isoelectric focusing * PCR * purification efficiency
    Kód oboru RIV: CB - Analytická chemie, separace
    Impakt faktor: 3.569, rok: 2010

    In this contribution Equine and Swine influenza viruses from the infected allantoic fluid from specific pathogen free embryonated chicken eggs were precipitaded by PEG 6000, sodium chloride and the impurities with different densities were removed in the sucrose gradients. The purification efficiency of this technique and the isoelectric points of tested viruses were determined by capillary isoelectric focusing, the purification efficiencies were compared with the results from PCR.
    Trvalý link: http://hdl.handle.net/11104/0181632