Počet záznamů: 1

Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in South Bohemia, the Czech Republic

  1. 1.
    0334941 - BC-A 2010 RIV NL eng J - Článek v odborném periodiku
    Ondráčková, Z. - Kváč, Martin - Sak, Bohumil - Květoňová, Dana - Rost, M.
    Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in South Bohemia, the Czech Republic.
    Veterinary Parasitology. Roč. 165, 1/2 (2009), s. 141-144 ISSN 0304-4017
    Grant CEP: GA ČR GP523/07/P117
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: Cryptosporidium spp. * cattle * slaughterhouses
    Kód oboru RIV: GJ - Choroby a škůdci zvířat, veterinární medicína
    Impakt faktor: 2.278, rok: 2009

    A total of 995 faecal samples from slaughtered dairy cattle was examined microscopically for the presence of Cryptosporidium spp. Nested PCR amplifying the partial SSU rRNA and GP60 genes of Cryptosporidium was performed on all positive and 200 randomly selected samples and the products were digested by SspI, VspI and MboII restriction enzymes. The highest prevalence of infection (18.2%) was in the animals in age group of 12-18 months. The sequence analyses of PCR-positive specimens identified 41 samples as C. andersoni (4.1%), 2 samples as C. bovis (0.2%), and 1 sample as C. parvum (0.1%). Cryptosporidium bovis was detected only in a group of cattle older than 18 months and C. parvum in heifer which was older than 14 months. Infection intensity was generally low. Sequence analysis of the GP60 gene showed that detected C. parvum belonged to the IIaA16G1R1 subtype. This is the first published report about subtyping of the Cryptosporidium GP60 gene from cattle in the Czech Republic.
    Trvalý link: http://hdl.handle.net/11104/0179548