Počet záznamů: 1

Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic

  1. 1.
    0334916 - BC-A 2010 RIV DE eng J - Článek v odborném periodiku
    Kváč, Martin - Sak, Bohumil - Hanzlíková, D. - Kotilová, J. - Květoňová, Dana
    Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic.
    Parasitology Research. Roč. 104, č. 2 (2009), s. 425-428 ISSN 0932-0113
    Grant CEP: GA ČR GP523/07/P117
    Výzkumný záměr: CEZ:AV0Z60220518
    Klíčová slova: Cryptosporidium spp. * slaughterhouse * pigs
    Kód oboru RIV: GJ - Choroby a škůdci zvířat, veterinární medicína
    Impakt faktor: 1.721, rok: 2009

    A total of 123 fecal samples of slaughtered finisher pigs and 21 sows from 14 farms were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. Positive samples were molecularly characterized by direct sequencing of partial SSU rRNA and GP60 partial genes as well as PCR-RFLP of SSU rRNA. Cryptosporidium oocysts were microscopically identified in 36 finishers (29%) and 2 sows (10%). Twenty one mono-infections of Cryptosporidium pig genotype II and 15 mixed-infection of Cryptosporidium pig genotype II and C. suis in finishers were found. Both sows were infected with the C. parvum subgenotype IIaA16G1R1, which is reported from pigs for the first time.
    Trvalý link: http://hdl.handle.net/11104/0179525