Počet záznamů: 1

Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases

  1. 1.
    0334373 - UOCHB-X 2010 RIV GB eng J - Článek v odborném periodiku
    Macíčková-Cahová, Hana - Hocek, Michal
    Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases.
    Nucleic Acids Research. Roč. 37, č. 22 (2009), s. 7612-7622 ISSN 0305-1048
    Grant CEP: GA ČR GA203/09/0317; GA MŠk LC512
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: functionalized DNA * restriction endonucleases * DNA polymerase * modified adenosines
    Kód oboru RIV: CC - Organická chemie
    Impakt faktor: 7.479, rok: 2009

    A set of base-modified 2'-deoxyadenosine derivatives was incorporated to diverse DNA sequences by primer extension and the influence of the modification on cleavage by diverse restriction endonucleases was studied. While 8-substituted adenine derivatives were well tolerated by the enzymes, the presence of 7-substituted 7-deazaadenine in the recognition sequence resulted in blocking of cleavage by some enzymes depending on the nature and size of the 7-substituent. The results are useful both for protection of some sequences from cleavage and for manipulation of functionalized DNA by restriction cleavage.
    Trvalý link: http://hdl.handle.net/11104/0179124