Počet záznamů: 1

Recovery of tubulin functions after freeze-dying in the presence of trehalose

  1. 1.
    0334042 - UMG-J 2010 RIV US eng J - Článek v odborném periodiku
    Dráberová, Eduarda - Sulimenko, Vadym - Sulimenko, Tetyana - Böhm, K. J. - Dráber, Pavel
    Recovery of tubulin functions after freeze-dying in the presence of trehalose.
    Analytical Biochemistry. Roč. 397, č. 1 (2010), s. 67-72 ISSN 0003-2697
    Grant CEP: GA AV ČR KAN200520701; GA MŠk LC545
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: Tubulin * Freeze-drying * Stability
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 3.236, rok: 2010

    We describe a novel method for freeze-drying of assembly-competent tubulin in the presence of a nonreducing sugar trehalose. Even after prolonged storage at ambient temperature, rehydrated tubulin is capable of binding antimitotic drugs and assembling to microtubules that bind microtubule-associated proteins in the usual way.
    Trvalý link: http://hdl.handle.net/11104/0178877