Počet záznamů: 1  

Zebrafish kidney stromal cell lines support multilineage hematopoiesis

  1. 1. 0333657 - UMG-J 2010 RIV US eng J - Článek v odborném periodiku
    Stachura, D.L. - Reyes, J.R. - Bartůněk, Petr - Paw, B.H. - Zon, L.I. - Traver, D.
    Zebrafish kidney stromal cell lines support multilineage hematopoiesis.
    Blood. Roč. 114, č. 2 (2009), s. 279-289 ISSN 0006-4971
    Grant CEP: GA MŠk(CZ) LC06077
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: Stromal cell line * hematopoietic progenitor cells * zebrafish growth factors
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 10.555, rok: 2009

    To enable more precise analyses of zebrafish hematopoietic progenitor cells, we have created zebrafish kidney stromal (ZKS) cell lines. Culture of kidney marrow with ZKS cells results in the maintenance and expansion of hematopoietic precursor cells. Hematopoietic growth is dependent upon ZKS cells, and we show that ZKS cells express many growth factors previously demonstrated to be important in maintaining mammalian hematopoietic cells. In the absence of exogenous growth factors, ZKS cells maintain early hematopoietic precursors and support differentiation of lymphoid and myeloid cells. With the addition of zebrafish erythropoietin, ZKS cells also support the differentiation of erythroid precursors. The development of robust in vitro assays now provide the means to track defined, functional outcomes for prospectively isolated blood cell subsets in the zebrafish.
    Trvalý link: http://hdl.handle.net/11104/0178596