Počet záznamů: 1

Hemoglobin digestion in blood-feeding ticks: mapping of multipeptidase pathway by functional proteomics

  1. 1.
    0333131 - UOCHB-X 2010 RIV US eng J - Článek v odborném periodiku
    Horn, Martin - Nussbaumerová, Martina - Šanda, Miloslav - Kovářová, Zuzana - Srba, Jindřich - Franta, Zdeněk - Sojka, Daniel - Bogyo, M. - Caffrey, C. R. - Kopáček, Petr - Mareš, Michael
    Hemoglobin digestion in blood-feeding ticks: mapping of multipeptidase pathway by functional proteomics.
    Chemistry & Biology. Roč. 16, č. 10 (2009), s. 1053-1063 ISSN 1074-5521
    Grant CEP: GA ČR GA206/06/0865; GA AV ČR IAA600960910; GA MŠk(CZ) LC06009; GA AV ČR KJB400550516
    Výzkumný záměr: CEZ:AV0Z40550506; CEZ:AV0Z60220518
    Klíčová slova: peptidases * active-site probes * hemoglobinolysis * tick digestion * Ixodes ricinus
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 6.523, rok: 2009

    Hemoglobin digestion is an essential process for blood-feeding parasites. We deconvoluted the hemoglobinolytic cascade in the tick Ixodes ricinus, a vector of Lyme disease and tick-borne encephalitis. A network of digestive peptidases was demonstrated through imaging with specific activity-based probes and activity profiling. Selective inhibitors were applied to dissect the roles of the individual peptidases and determine the peptidase-specific cleavage map of the hemoglobin molecule. Because of their central function in nutrition of the parasite, the identified enzymes are potential targets to developing novel anti-tick vaccines that limit parasite survival and disease transmission.
    Trvalý link: http://hdl.handle.net/11104/0178196