Počet záznamů: 1

Recombinant immobilized rhizopuspepsin as a new tool for protein digestion in hydrogen/deuterium exchange mass spectrometry

  1. 1.
    0330788 - MBU-M 2010 RIV GB eng J - Článek v odborném periodiku
    Rey, M. - Man, Petr - Brandolin, G. - Forest, E. - Pelosi, L.
    Recombinant immobilized rhizopuspepsin as a new tool for protein digestion in hydrogen/deuterium exchange mass spectrometry.
    Rapid Communications in Mass Spectrometry. Roč. 23, č. 21 (2009), s. 3431-3438 ISSN 0951-4198
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: mass spectrometry * recombinant immobilized rhizopuspepsin * protease
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 2.695, rok: 2009

    This paper reports on expression, ioslation and testing of protease type XVIII from Rhizopus sp. Recombinant protease proved to be much more efficient than the commercially availabe crude extract. Moreover it showed excellent cleavage specifity and efficiency when immobilized to the POROS resin. The use of this protease enhance spatial resolution achievable in hydrogen/deuterium exchange coupled to mass spectrometry
    Trvalý link: http://hdl.handle.net/11104/0005489