Počet záznamů: 1

Stepwise DNA Methylation Changes Are Linked to Escape from Defined Proliferation Barriers and Mammary Epithelial Cell Immortalization

  1. 1.
    0329667 - BC-A 2010 RIV US eng J - Článek v odborném periodiku
    Novák, Petr - Jensen, T.J. - Garbe, J. - Sampfer, M.R. - Futscher, B. W.
    Stepwise DNA Methylation Changes Are Linked to Escape from Defined Proliferation Barriers and Mammary Epithelial Cell Immortalization.
    Cancer Research. Roč. 69, č. 12 (2009), s. 5251-5258 ISSN 0008-5472
    Výzkumný záměr: CEZ:AV0Z50510513
    Klíčová slova: Epigenetics * Breast Cancer * DNA methylation
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 7.543, rok: 2009

    We analyzed genome-wide DNA methylation patterns in an isogenic human mammary epithelial cell (HMEC) culture model of transformation. To acquire immortality and malignancy, the cultured finite lifespan HMEC must overcome two distinct proliferation barriers. Our analysis of HMEC transitioning from finite lifespan to malignantly transformed showed that aberrant DNA methylation changes occur in a stepwise fashion early in the transformation process. The first aberrant DNA methylation step coincides with overcoming stasis, and results in few to hundreds of changes, depending on how stasis was overcome. A second step coincides with immortalization and results in hundreds of additional DNA methylation changes regardless of the immortalization pathway. A majority of these DNA methylation changes are also found in malignant breast cancer cells.
    Trvalý link: http://hdl.handle.net/11104/0175639