0488261 - BC 2018 RIV CZ eng L - Prototyp, funkční vzorek
Holzer, Astrid S. - Fiala, Ivan - Born-Torrijos, AnaTesting parasiticidal and immunomodulatory substances to combat myxozoan infections in aquaculture using novel in vitro and in vivo models.
Interní kód: TG02010034_Rybomorky_Holzer ; 2017
Technické parametry: The presented functional sample represents a continuous in vivo culture and transmission system for blood stages of the myxozoan parasite S. molnari. S. molnari blood stages (SMBS) are transmitted to specific pathogen-free (SPF) fish via intraperitoneal injection and are harvested from host blood after 28 days, when parasite levels are at their peak. SMBS are then collected and used to infect a new lot of SPF carp. The aim of this process is 1. To be able to produce large numbers of SMBS throughout the year, independent from environmental conditions (frozen ponds during the winter months), 2. To work in a ‘clean’ culture system devoid of co-infections with other parasites, fungi or bacteria, usually present in ponds, and 3. To be able to test the effect of potentially parasiticidal or immunomodulatory substances in an in vivo model, aiming at essential reduction/removal of myxozoan proliferative stages. If effective, such substances could then be included into anti- parasitic diets for fish, with S. molnari serving as a model for other myxozoan infections in fish in aquaculture systems worldwide. This is of particular importance as a treatment against myxozoans in fish destined for human consumption is presently unavailable.
Ekonomické parametry: Předpokládaným výstupem je prodej licence know-how a z toho plynoucí licenční poplatky pro výzkumnou instituci. Odhad zisku pro výzkumnou instituci je 300 000,- Kč.
Grant CEP: GA TA ČR(CZ) TG02010034
Institucionální podpora: RVO:60077344
Klíčová slova: in vitro * in vivo * Myxozoa
Obor OECD: GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
Trvalý link: http://hdl.handle.net/11104/0282870
