0420833 - MBÚ 2014 DE eng J - Článek v odborném periodiku
Kaplan, Ondřej - Vejvoda, Vojtěch - Plíhal, O. - Pompach, P. - Kavan, D. - Bojarová, Pavla - Bezouška, K. - Macková, M. - Cantarella, M. - Jirků, V. - Křen, Vladimír - Martínková, LudmilaErratum to: Purification and characterization of a nitrilase from Aspergillus niger.
Applied Microbiology and Biotechnology. Roč. 97, č. 8 (2013), s. 3745-3746. ISSN 0175-7598. E-ISSN 1432-0614
Institucionální podpora: RVO:61388971
Klíčová slova: nitrilase * Aspergillus niger
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.811, rok: 2013
The previous article reported on the biochemical characterization of a nitrilase purified from Aspergillus niger K10. The amino acid sequence of this enzyme was recently analyzed by mass spectroscopy which revealed that the N-terminal sequence reported in Fig. 3A (by KB) in the previous article was incorrect. This N-terminal sequence (XAPVLKKYKAAXVNXE), which was highly homologous to those of a number of hypothetical proteins in genus Aspergillus (Aspergillus fumigatus Af29, Aspergillus oryzae, Aspergillus nidulans FGSC A4) did not belong to the enzyme purified and characterized in the previous article. Mass spectrum analyses of this enzyme were recently performed as follows. Briefly, the peptides were extracted after in gel digestion of the enzyme with trypsin and analyzed by MALDI-ToF MS using Bruker Biflex IV (Bruker Daltonics, Germany). Alternatively, the peptides were analyzed by using UHPLC Dionex Ultimate3000 RSLC nano (Dionex, Germany) equipped with a ESI-Q-ToF Maxis Impact (Bruker Daltonics, Germany) mass spectrometer. Spectra were interpreted using Mascot software (Matrix Science, UK)
Trvalý link: http://hdl.handle.net/11104/0227388
