Microfluidic platform for cell lysis: Towards single-molecule analysis in a free solution

0576903 - ÚIACH 2024 RIV VN eng C - Konferenční příspěvek (zahraniční konf.)
Křivánková, Jana - Hlaváček, Antonín
Microfluidic platform for cell lysis: Towards single-molecule analysis in a free solution.
8th CASEAN Proceedings. Vinh City: Publishing House for Science and Technology, 2023, s. 34-40. ISBN 978-604-357-225-4.
[The Academic Conference On Natural Science For Young Scientists, Master And PhD Students From Asean Countries /8./. Vinh City (VN), 27.08.2023-30.08.2023]
Grant CEP: GA ČR(CZ) GA21-03156S
Grant ostatní: AV ČR(CZ) VAST-22-01
Program: Bilaterální spolupráce
Institucionální podpora: RVO:68081715
Klíčová slova: droplet microfluidics * polydimethylsiloxane chip * cell lysis
Obor OECD: Analytical chemistry

We proposed a PDMS droplet-based microfluidic chip, with passive cell encapsulation, passive mixing, and incubation part. Discrete droplets with at least one cell per droplet in a regulated fashion of about 1Hz were formed and the cell-lysis concept using detergent Triton X-100 was optimized. The examined approach has essential benefits for cell manipulation, separation, and analysis of the droplet content.
Trvalý link: https://hdl.handle.net/11104/0346292