Počet záznamů: 1
Detection and Quantification of Carbohydrate-Deficient Transferrin by MALDI-Compatible Protein Chips Prepared by Ambient Ion Soft Landing
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SYSNO ASEP 0496286 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Detection and Quantification of Carbohydrate-Deficient Transferrin by MALDI-Compatible Protein Chips Prepared by Ambient Ion Soft Landing Tvůrce(i) Darebná, Petra (MBU-M)
Špička, J. (CZ)
Kučera, R. (CZ)
Topolčan, O. (CZ)
Navrátilová, E. (CZ)
Růžička, V. (CZ)
Volný, Michael (MBU-M) ORCID
Novák, Petr (MBU-M) RID, ORCID
Pompach, Petr (MBU-M) RID, ORCIDZdroj.dok. Clinical Chemistry. - : Oxford University Press - ISSN 0009-9147
Roč. 64, č. 9 (2018), s. 1319-1326Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova MASS-SPECTROMETRY ; ALCOHOL-CONSUMPTION ; SERUM TRANSFERRIN Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP LM2015043 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LH15010 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LD15089 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000448300800011 EID SCOPUS 85052469905 DOI 10.1373/clinchem.2017.285452 Anotace BACKGROUND: Transferrin is synthetized in the liver and is the most important iron-transport carrier in the human body. Severe alcohol consumption leads to alterations in glycosylation of transferrin. Mass spectrometry can provide fast detection and quantification of transferrin isoforms because they have different molecular masses. In this study, we used antibody chips in combination with MALDI-TOF MS for the detection and quantification of transferrin isoforms.
METHODS: Protein chips were prepared by functionalization of indium tin oxide glass using ambient ion soft landing of electrosprayed antitransferrin antibody. Two microliters of patient serum was applied on the antibody-modified spots, and after incubation, washing, and matrix deposition, transferrin isoforms were detected by MALDI-TOF MS. Peak intensities of each transferrin form were used to calculate total carbohydrate-deficient transferrin (CDT). The CDT values obtained by the MALDI chip method were compared with the results obtained by a standard capillary electrophoresis (CE).
RESULTS: The chip-based MALDI-TOF MS method was used for enrichment and detection of CDT from human serum. A sample cohort from 186 patients was analyzed. Of these samples, 44 were positively identified as belonging to alcoholic patients, whereas 142 were negative by the MALDI chip approach. The correlation of the data obtained by the CE and the chip-based MALDI was r = 0.986, 95% CI.
CONCLUSIONS: Functionalized MALDI chips modified by antitransferrin antibody prepared by ambient ion soft landing were successfully used for detection and quantification of CDT from human sera. (c) 2018 American Association for Clinical Chemistry.Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
Počet záznamů: 1