Počet záznamů: 1
Communication between N terminus and loop2 tunes Orai activation
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SYSNO ASEP 0489833 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Communication between N terminus and loop2 tunes Orai activation Tvůrce(i) Fahrner, M. (AT)
Pandey, Saurabh Kumar (MBU-M) ORCID
Muik, M. (AT)
Traxler, L. (AT)
Butorac, C. (AT)
Stadlbauer, M. (AT)
Zayats, Vasilina (MBU-M) ORCID
Křížová, A. (AT)
Plenk, P. (AT)
Frischauf, I. (AT)
Schindl, R. (AT)
Gruber, H.J. (AT)
Hinterdorfer, P. (AT)
Ettrich, Rüdiger (MBU-M)
Romanin, C. (AT)
Derler, I. (AT)Zdroj.dok. Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258
Roč. 293, č. 4 (2018), s. 1271-1285Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova atomic force microscopy (AFM) ; calcium release-activated calcium channel protein 1 (ORAI1) ; electrophysiology Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP LTC17069 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000423515000014 EID SCOPUS 85041209005 DOI 10.1016/j.saa.2017.12.021 Anotace The CRAC channel gating involves the binding of STIM1 C-terminus to cytosolic N- and C-termini of Orai. The C-termini of Orai is the main binding partner however N-termini also have been found necessary for Orai channel function. Despite a highly conserved ETON region Orai1 and Orai3 differ in length of the ETON region. Our aim here was to study the reasons for different behavior of these two isoforms and to find the differences in structural requirement for gating and function. While there is no interaction between the N-terminus and loop2 in Orai3, we predict that interactions between loop2 and Orai1 N-terminus results into masking the STIM1 coupling sites which are located either at N-terminus or loop2 or both, thus loop2 either directly or allosterically affects the STIM1-Orai coupling in an isoform specific manner. Thus along with the requirement of conserved ETON region a fine tuning between N-termini and loop2 conformations has an essential role in maintaining permissive conformation of channel and store-operated function of Orai channel.
Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
Počet záznamů: 1