Počet záznamů: 1
Ultra-rapid auxin metabolite profiling for high-throughput mutant screening in Arabidopsis
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SYSNO ASEP 0489589 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Ultra-rapid auxin metabolite profiling for high-throughput mutant screening in Arabidopsis Tvůrce(i) Pěnčík, Aleš (UEB-Q) ORCID, RID, SAI
Casanova-Sáez, R. (SE)
Pilařová, V. (CZ)
Žukauskaitė, Asta (UEB-Q) ORCID
Pinto, R. (FR)
Micol, J.L. (ES)
Ljung, K. (SE)
Novák, Ondřej (UEB-Q) RID, ORCID, SAICelkový počet autorů 8 Zdroj.dok. Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 69, č. 10 (2018), s. 2569-2579Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Arabidopsis thaliana ; auxin ; metabolite profiling ; multivariate data analysis ; mutant ; screening Vědní obor RIV ED - Fyziologie Obor OECD Plant sciences, botany CEP GJ17-21581Y GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000431251900011 EID SCOPUS 85046280384 DOI 10.1093/jxb/ery084 Anotace Auxin (indole-3-acetic acid, IAA) plays fundamental roles as a signalling molecule during numerous plant growth and development processes. The formation of local auxin gradients and auxin maxima/minima, which is very important for these processes, is regulated by auxin metabolism (biosynthesis, degradation, and conjugation) as well as transport. When studying auxin metabolism pathways it is crucial to combine data obtained from genetic investigations with the identification and quantification of individual metabolites. Thus, to facilitate efforts to elucidate auxin metabolism and its roles in plants, we have developed a high-throughput method for simultaneously quantifying IAA and its key metabolites in minute samples ( < 10 mg FW) of Arabidopsis thaliana tissues by in-tip micro solid-phase extraction and fast LC-tandem MS. As a proof of concept, we applied the method to a collection of Arabidopsis mutant lines and identified lines with altered IAA metabolite profiles using multivariate data analysis. Finally, we explored the correlation between IAA metabolite profiles and IAA-related phenotypes. The developed rapid analysis of large numbers of samples ( > 100 samples d1) is a valuable tool to screen for novel regulators of auxin metabolism and homeostasis among large collections of genotypes. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2019
Počet záznamů: 1